Iron overload adversely effects bone marrow haematogenesis via SIRT-SOD2-mROS in a process ameliorated by curcumin

Abstract Background Iron overload, which is common in patients with haematological disorders, known to have a suppressive effect on haematogenesis. However, the mechanism for this still unclear. The antioxidant curcumin has been reported protect against iron overload-induced bone marrow damage through an as-yet-unknown mechanism. Methods We established overload cell and mouse models. Mitochondrial reactive oxygen species (mROS) levels, autophagy levels SIRT3/SOD2 pathway were examined models of overload. Results was shown depress haematogenesis induce mitochondrion-derived superoxide anion-dependent autophagic death. loading decreased SIRT3 protein expression, promoted increase SOD2, led elevation mROS. Overexpression reversed these effects. Curcumin treatment ameliorated peripheral blood cells generation, enhanced activity, SOD2 acetylation, inhibited mROS production, suppressed loading-induced autophagy. Conclusions Our results suggest that exerts protective by reducing mROS-stimulated death manner dependent pathway.